ROCK1 CLIA Kit for Human(Homo sapiens) | EIAab.comROCK1 CLIA Kit for Human(Homo sapiens)

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ROCK1 (Gene name),

Rho-associated protein kinase 1 (Protein name ), ROCK1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

ROCK1;

Protein name:

Rho-associated protein kinase 1;

Alternative:

Rho-associated, coiled-coil-containing protein kinase 1;Renal carcinoma antigen NY-REN-35;p160 ROCK-1(p160ROCK);Rho-associated, coiled-coil-containing protein kinase I(ROCK-I);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.

Function:

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation.

Subcellular Location:

Cytoplasm Cytoplasm cytoskeleton centrosome centriole Golgi apparatus membrane Peripheral membrane protein Cell projection bleb Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.

Protein Attributes:

Sequence length:

1354

Sequence:

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1050:

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1150:

1200:

1250:

1300:

1350:

1354:

GKTS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

Q13464

MIM:

601702

UniGene:

Hs.306307

String:

Q13464

KEGG:

hsa:6093

Pfam:

PF02185

STRING:

9606.ENSP00000382697

Uniprot:

Q13464

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ELISA Kit for Human Rho-associated protein kinase 1

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E15189h

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ELISA Kit for Human Rho-associated protein kinase 1

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E15189r

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96T

ELISA Kit for Human Rho-associated protein kinase 1

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ELISA Kit for Human Rho-associated protein kinase 1

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E15189m

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ELISA Kit for Human Rho-associated protein kinase 1

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E15189b

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96T

CLIA Kit for Human Rho-associated protein kinase 1

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U15189r

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96T

CLIA Kit for Human Rho-associated protein kinase 1

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U15189h

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96T

CLIA Kit for Human Rho-associated protein kinase 1

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CLIA Kit for Human Rho-associated protein kinase 1

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U15189b

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96T

CLIA Kit for Human Rho-associated protein kinase 1

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U15189m

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Polyclonal Antibody for Human Rho-associated protein kinase 1

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P15189Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human Rho-associated protein kinase 1

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P15189Rb-h

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Polyclonal Antibody for Human Rho-associated protein kinase 1

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P15189Rb-r

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Polyclonal Antibody for Human Rho-associated protein kinase 1

Monoclonal Antibody for Human Rho-associated protein kinase 1

Protein for Human Rho-associated protein kinase 1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase."
Ishizaki T. , Maekawa M. , Fujisawa K. , Okawa K. , Iwamatsu A. , Fujita A. , Watanabe N. , Saito Y. , Kakizuka A. , Morii N. , Narumiya S.
EMBO J.15:1885-1893(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 187-195; 281-288; 465-473; 818-828; 885-893 AND 934-945;FUNCTION;PHOSPHORYLATION;INTERACTION WITH RHOA;SUBCELLULAR LOCATION;TISSUE SPECIFICITY
tissue: Leukemia.
2.

NHLBI resequencing and genotyping service (RS&G)
Submitted (2007-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
4.

Bienvenut W.V. , Ramsay A. , Leung H.Y.
Submitted (2009-03) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-13; 85-94; 327-334; 405-415 AND 546-563;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY
tissue: Embryonic kidney.
5.

Totoki Y. , Toyoda A. , Takeda T. , Sakaki Y. , Tanaka A. , Yokoyama S. , Ohara O. , Nagase T. , Kikuno R.F.
Submitted (2005-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 323-1027
tissue: Brain.
6.

"Different molecular mechanisms for Rho family GTPase-dependent, Ca2+-independent contraction of smooth muscle."
Van Eyk J.E. , Arrell D.K. , Foster D.B. , Strauss J.D. , Heinonen T.Y. , Furmaniak-Kazmierczak E. , Cote G.P. , Mak A.S.
J. Biol. Chem.273:23433-23439(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ROLE IN SMOOTH MUSCLE CONTRACTION
7.

"Antigens recognized by autologous antibody in patients with renal-cell carcinoma."
Scanlan M.J. , Gordan J.D. , Williamson B. , Stockert E. , Bander N.H. , Jongeneel C.V. , Gure A.O. , Jaeger D. , Jaeger E. , Knuth A. , Chen Y.-T. , Old L.J.
Int. J. Cancer83:456-464(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN
tissue: Renal cell carcinoma.
8.

"Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase."
Maekawa M. , Ishizaki T. , Boku S. , Watanabe N. , Fujita A. , Iwamatsu A. , Obinata T. , Ohashi K. , Mizuno K. , Narumiya S.
Science285:895-898(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH LIMK1 AND LIMK2;INHIBITION BY Y-27632
9.

"Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop."
Ohashi K. , Nagata K. , Maekawa M. , Ishizaki T. , Narumiya S. , Mizuno K.
J. Biol. Chem.275:3577-3582(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH LIMK1
10.

"Specific activation of LIM kinase 2 via phosphorylation of threonine 505 by ROCK, a Rho-dependent protein kinase."
Sumi T. , Matsumoto K. , Nakamura T.
J. Biol. Chem.276:670-676(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH LIMK2
11.

"Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing."
Sebbagh M. , Renvoize C. , Hamelin J. , Riche N. , Bertoglio J. , Breard J.
Nat. Cell Biol.3:346-352(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CLEAVAGE BY CASPASE-3;MUTAGENESIS OF ASP-1113;INTERACTION WITH PPP1R12A;FUNCTION
12.

"The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway."
Ward Y. , Yap S.-F. , Ravichandran V. , Matsumura F. , Ito M. , Spinelli B. , Kelly K.
J. Cell Biol.157:291-302(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GEM
13.

"RhoE binds to ROCK I and inhibits downstream signaling."
Riento K. , Guasch R.M. , Garg R. , Jin B. , Ridley A.J.
Mol. Cell. Biol.23:4219-4229(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RHOE AND PPP1R12A;SUBCELLULAR LOCATION
14.

"Rocks: multifunctional kinases in cell behaviour."
Riento K. , Ridley A.J.
Nat. Rev. Mol. Cell Biol.4:446-456(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
15.

"Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis."
Morita E. , Sandrin V. , Chung H.Y. , Morham S.G. , Gygi S.P. , Rodesch C.K. , Sundquist W.I.
EMBO J.26:4215-4227(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TSG101
16.

"ROCK1 phosphorylates and activates zipper-interacting protein kinase."
Hagerty L. , Weitzel D.H. , Chambers J. , Fortner C.N. , Brush M.H. , Loiselle D. , Hosoya H. , Haystead T.A.
J. Biol. Chem.282:4884-4893(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH DAPK3
17.

"The diaphanous-related formin FHOD1 associates with ROCK1 and promotes Src-dependent plasma membrane blebbing."
Hannemann S. , Madrid R. , Stastna J. , Kitzing T. , Gasteier J. , Schoenichen A. , Bouchet J. , Jimenez A. , Geyer M. , Grosse R. , Benichou S. , Fackler O.T.
J. Biol. Chem.283:27891-27903(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH FHOD1;SUBCELLULAR LOCATION
18.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1105;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation."
Shao J. , Welch W.J. , Diprospero N.A. , Diamond M.I.
Mol. Cell. Biol.28:5196-5208(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PFN1
20.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
21.

"Identification of ROCK1 as an upstream activator of the JIP-3 to JNK signaling axis in response to UVB damage."
Ongusaha P.P. , Qi H.H. , Raj L. , Kim Y.B. , Aaronson S.A. , Davis R.J. , Shi Y. , Liao J.K. , Lee S.W.
Sci. Signal.1:RA14-RA14(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH JIP3
22.

"Inhibition of Rho-dependent kinases ROCK I/II activates VEGF-driven retinal neovascularization and sprouting angiogenesis."
Kroll J. , Epting D. , Kern K. , Dietz C.T. , Feng Y. , Hammes H.P. , Wieland T. , Augustin H.G.
Am. J. Physiol.296:H893-H899(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
23.

"ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells."
Wang Y. , Zheng X.R. , Riddick N. , Bryden M. , Baur W. , Zhang X. , Surks H.K.
Circ. Res.104:531-540(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PPP1R12A
24.

"Distinct roles for ROCK1 and ROCK2 in the regulation of keratinocyte differentiation."
Lock F.E. , Hotchin N.A.
PLoS ONE4:E8190-E8190(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
25.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1105;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
26.

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-647;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
27.

"Morgana/chp-1, a ROCK inhibitor involved in centrosome duplication and tumorigenesis."
Ferretti R. , Palumbo V. , Di Savino A. , Velasco S. , Sbroggio M. , Sportoletti P. , Micale L. , Turco E. , Silengo L. , Palumbo G. , Hirsch E. , Teruya-Feldstein J. , Bonaccorsi S. , Pandolfi P.P. , Gatti M. , Tarone G. , Brancaccio M.
Dev. Cell18:486-495(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CHORDC1
28.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
29.

"Caspase-activated ROCK-1 allows erythroblast terminal maturation independently of cytokine-induced Rho signaling."
Gabet A.S. , Coulon S. , Fricot A. , Vandekerckhove J. , Chang Y. , Ribeil J.A. , Lordier L. , Zermati Y. , Asnafi V. , Belaid Z. , Debili N. , Vainchenker W. , Varet B. , Hermine O. , Courtois G.
Cell Death Differ.18:678-689(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;CLEAVAGE BY CASPASE-3
30.

"Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity."
Amano M. , Nakayama M. , Kaibuchi K.
Cytoskeleton67:545-554(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
31.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
32.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
33.

"Structural insights into the interaction of ROCKI with the switch regions of RhoA."
Dvorsky R. , Blumenstein L. , Vetter I.R. , Ahmadian M.R.
J. Biol. Chem.279:7098-7104(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 947-1015 IN COMPLEX WITH RHOA
34.

"The structure of dimeric ROCK I reveals the mechanism for ligand selectivity."
Jacobs M. , Hayakawa K. , Swenson L. , Bellon S. , Fleming M. , Taslimi P. , Doran J.
J. Biol. Chem.281:260-268(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 6-415;SUBUNIT
35.

"Patterns of somatic mutation in human cancer genomes."
Greenman C. , Stephens P. , Smith R. , Dalgliesh G.L. , Hunter C. , Bignell G. , Davies H. , Teague J. , Butler A. , Stevens C. , Edkins S. , O'Meara S. , Vastrik I. , Schmidt E.E. , Avis T. , Barthorpe S. , Bhamra G. , Buck G. , more...
Nature446:153-158(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASN-108; SER-773; PRO-1112; SER-1193; GLU-1217; GLN-1262 AND ARG-1264

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